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After taking a break, employees must wash their hands to ensure food safety and hygiene. This practice is critical because during breaks, hands may come into contact with various surfaces, food, or personal items that can introduce contaminants. Washing hands removes any potential pathogens or food residues that could be harmful if they were to come into contact with food or food preparation surfaces afterward. This step helps maintain a safe environment for both employees and customers, aligning with health department regulations and best practices in food handling.

The other options, while they might seem reasonable in a general food preparation context, do not specifically address the immediate need for hand hygiene after a break. For instance, putting on fresh gloves may be necessary when switching tasks, but it does not replace the need for hand washing. Resting for an additional five minutes or changing uniforms are not essential practices related to food safety following a break.